No.
Here is a proof. Suppose $G$ is a weighted undirected graph such that for every cut of the graph, there is not a unique lightest edge crossing the cut.
Let $M$ be a (or "the" in case it is unique) minimum spanning tree of $G$. Let $v$ be a leaf node of $M$. Consider the cut $\{\{v\},V\setminus \{v\}\}$ of $G$. Since $M$ is spanning, it contains an edge $m$ that crosses the cut. There are at least two edges of the minimum weight that cross the cut. Suppose one of them is $c_1\not=m$. Let graph $M'$ be the same as $M$ but with $m$ replaced by $c_1$. It is a routine to show that $M'$ is also an MST. So we know $G$ has two different $MST$s, $M$ and $M'$.
This negative conclusion can be seen as an corollary of the characterization theorem of the uniqueness of MST as stated in this answer. Here is the relevant part of that theorem.
(Uniqueness of MST by cut) Let $G$ be a weighted undirected graph. $G$ has a unique MST if and only if every edge is either the unique edge of minimum weight crossing some cut or never an edge of minimum weight to cross any cut.
Now let us assume $G$ is a graph of at least 2 nodes such that for every cut of the graph, there is not a unique light edge crossing the cut. Let $C$ be a cut of $G$. Let $e$ be the edge of minimum weight in the cut-set of $C$. Because $e$ is not the unique edge of minimum weight to cross any cut, we see that $G$ cannot have a unique MST.