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The fact that P ≠ NP does not preclude the possibility that NP = co-NP, in which case NP ∩ co-NP = NP. So to further the discussion, let us assume that NP ≠ co-NP. In that case, Corollary 9 in Schöning's A uniform approach to obtain diagonal sets in complexity classes shows that there exists some language in NP – co-NP which is NP-intermediate. So NPI strictly contains NP ∩ co-NP (note that every language in NP ∩ co-NP is in P ∪ NPI). This is your option (4).